Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of Live Cell Imaging

Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of Live Cell Imaging

Author(s): Barry R. Masters

Published: 2006

https://doi.org/10.1117/3.660403

PDF ISBN: 9780819478344 | Print ISBN: 9780819461186

DESCRIPTION

This text guides you through the principles and practical techniques of confocal and multiphoton microscopy. It also describes the historical connections and parallel inventions that resulted in modern techniques of live cell imaging and their use in biology and medicine. You will find comparisons of different types of confocal and multiphoton microscopes, solutions to the problems one would encounter when using various microscopic techniques, tips on selecting equipment, and an extensive annotated bibliography of additional resources.

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Front Matter

CHAPTER 1.

A Brief History of the Microscope and Its Significance in the Advancement of Biology and Medicine

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CHAPTER 2.

The Optical Microscope: Its Principles, Components, and Limitations

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CHAPTER 3.

Abbe Theory of Image Formation and Diffraction of Light in Transmitted Light Microscopes

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CHAPTER 4.

Optical Resolution and Resolving Power: What It Is, How to Measure It, and What Limits It

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CHAPTER 5.

Techniques That Provide Contrast

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CHAPTER 6.

Early Antecedents of Confocal Microscopy

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CHAPTER 7.

Optical Sectioning (Depth Discrimination) with Different Scanning Techniques: The Beginnings of Confocal Microscopy

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CHAPTER 8.

The Development of Scanning-Slit Confocal Systems for Imaging Live Cells, Tissues, and Organs

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CHAPTER 9.

The Components of a Confocal Microscope

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CHAPTER 10.

The Development of Nonlinear Spectroscopy and Microscopy

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CHAPTER 11.

Multiphoton Excitation Microscopy

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CHAPTER 12.

Theory and Instrumentation of Multiphoton Excitation Microscopy

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CHAPTER 13.

Remaining Problems, Limitations, and Their Partial Solutions

CHAPTER 14.

Speculation on Future Directions for Confocal and Multiphoton Excitation Microscopy

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CHAPTER 15.

Safety and Cleanliness Considerations

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Back Matter

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