Over the last decade, the field of femtosecond eye surgery has expanded rapidly, supporting the advantage of combined high ablation precision and minimized collateraltissue effects. One of the most promising applications for femtosecond laser eye surgery has been corneal surgery, namely laser in-situ keratomileusis (LASIK) surgery, where the high-pressure laser plasma non-thermally dissociates the dense corneal tissue thereby enabling lamellar cornea procedures with minimized side effects and subsequent excimer laser shapes the cornea surface. Millions of people worldwide had been patient for LASIK surgery and benefitted from such new technology. More and more interests were shown on this surgery and people want to know more about this surgery itself and research beneath it. LASIK surgery includes three parts: wavefront detection, femtosecond laser flap creation and excimer laser cornea correction. This special issue focuses on femtosecond laser assisted LASIK surgery, content includes basic research for femtosecond laser eye surgery and femtosecond laser eye tissue imaging.