An Engineering Introduction to Biotechnology

Author(s): J. Patrick Fitch

Published: 2002

PDF ISBN: 9780819478634 | Print ISBN: 9780819444974

DESCRIPTION

This tutorial will help technical professionals in optics determine whether their technologies have potential application in the life sciences. It also is useful as a 'prep class' for more detailed books on biology and biotechnology, filling the gap between fundamental and high-level approaches.
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Front Matter

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CHAPTER 1.

Basic Biology

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1.1 Life
1.2 Cells—Archaea, Eukarya, and Bacteria
1.2.1 From DNA to protein
1.3 Viruses, Viroids, and Prions

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CHAPTER 2.

Nucleic Acids as the Blueprint

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2.1 Genetics to Genomics
2.2 DNA
2.3 RNA
2.4 How DNA Codes for Protein
2.4.1 Transcription
2.5 Genetic Regulation
2.6 Mutations and Disease

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CHAPTER 3.

Manipulating Nucleic Acids and Proteins

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3.1 Sizing DNA and Proteins
3.2 Blots
3.3 Cutting DNA and Protein
3.3.1 Restriction enzymes
3.3.2 Protein digests
3.4 Copying DNA
3.5 Genetic Engineering
3.5.1 Transformation
3.5.2 Bacteriophage cloning systems
3.5.3 Cosmid cloning systems
3.5.4 Artificial chromosome cloning systems
3.6 Protein Expression
3.6.1 Using cells to express proteins
3.6.2 Natural optical signatures
3.6.3 In vitro protein production

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CHAPTER 4.

An Integrated Approach for Biological Discovery

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CHAPTER 5.

DNA Sequencing

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5.1 Sequencing Approaches
5.2 Instruments
5.2.1 Optical detection subsystem example
5.3 Automation

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CHAPTER 6.

Detecting Nucleic Acids

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6.1 Environmental Detection Chips
6.2 Gene Expression Microarrays
6.3 Multiaffinity Assays
6.3.1 Hybrid DNA chip
6.3.2 Bead-based flow cytometry for detection

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CHAPTER 7.

Protein Structure

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7.1 Nuclear Magnetic Resonance
7.2 X-ray Crystallography
7.3 Computational Prediction of Structure

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Units and Measures

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Nonscientific Issues

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Back Matter

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