Optical Blood Flow Measurement in Microcirculatory Systems

Optical Blood Flow Measurement in Microcirculatory Systems
Author(s):    Laura Sironi; Laura D’Alfonso; Pablo Blinder; Margaux Bouzin; Nicolo Giovanni Ceffa; Maddalena Collini; Giuseppe Chirico
Published:   2016
DOI:             10.1117/3.2256729
eISBN: 9781510605008
Description:

This Spotlight reviews non-invasive optical methods to determine the blood flow velocity in microcirculation, with an emphasis on the most recent developments, that may be routinely applied in cellular biology laboratories. The methods covered in this book include both scanning and whole-field optical imaging techniques based on endogenous or exogenous (fluorescent) contrast agents for plasma and red-blood-cell visualization, such as laser Doppler velocimetry, laser speckle contrast imaging, diffuse correlation spectroscopy, particle image velocimetry, and two-photon microscopy. Several methods have been developed to quantify the blood flow velocity within microvessels by exploiting the cross-correlation concept applied to optical scanning microscopes: dual-foci cross-correlation, spatio-temporal image correlation spectroscopy, line-scanning techniques, and a recently developed single raster-scanned xy-image technique (FLICS). The basic operational principles, the advantages and disadvantages, and the technical issues of these methods will be discussed and compared, and their applications in biomedical research will be presented.

Please Wait... Processing your request... Please Wait.

Microcirculation, a complex network of small vessels consisting of arterioles, capillaries, and venules, with a typical diameter ranging from 5 to 50 μm, delivers blood, nutrients, and signaling molecules to tissues and organs, playing a crucial role in their maintenance and hemodynamics. Moreover, microcirculation impairments and dysfunctions are involved in a variety of pathological processes, including cardiovascular diseases, diabetes, excessive angiogenesis, and tumors.

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.

Your library does not currently subscribe to eBooks on the SPIE Digital Library. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.