Separation and detection of cancer-derived exosome using anti-CD63 Ab-MNPs and aptamer-PDA liposomes

Yun Kyung Jung; Min Hyeong Son

doi:10.1117/12.2609421

4 March 2022 Separation and detection of cancer-derived exosome using anti-CD63 Ab-MNPs and aptamer-PDA liposomes

Yun Kyung Jung, Min Hyeong Son

Proceedings Volume PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV; PC119790E (2022) https://doi.org/10.1117/12.2609421
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Exosomes have received much attention as biomarkers for the diagnosis and treatment of various diseases such as diabetic cardiomyopathy and cancer. However, it remains an important challenge to quickly and precisely isolate and detect exosomes from various body fluids. Therefore, we selectively separated exosomes using anti-CD63 antibody-conjugated magnetic nanoparticles (anti-CD63 Ab-MNPs). And, to detect the exosomes optically, an aptamer-conjugated PDA liposome (Apt-PDA) was developed to target EpCAM overexpressed on the surface of cancer-derived exosome. The isolated exosomes were detected through the colorimetric change and the red fluorescence of PDA. We demonstrated simultaneous separation and detection of exosomes by anti-CD63 Ab-MNPs and anti-EpCAM Apt-PDA.

Conference Presentation

Citation Download Citation

Yun Kyung Jung and Min Hyeong Son "Separation and detection of cancer-derived exosome using anti-CD63 Ab-MNPs and aptamer-PDA liposomes", Proc. SPIE PC11979, Frontiers in Biological Detection: From Nanosensors to Systems XIV, PC119790E (4 March 2022); https://doi.org/10.1117/12.2609421

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