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6 March 2023 New method for traceable refractive index determination by measuring the angle of minimum deviation
Martine Kuiper, Richard Koops, Rienk Nieuwland, Ton G. van Leeuwen, Edwin van der Pol
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Proceedings Volume PC12370, Design and Quality for Biomedical Technologies XVI; PC1237002 (2023) https://doi.org/10.1117/12.2650587
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Refractive index (RI) measurements are highly dependent on environmental conditions, which are often not reported, resulting in non-traceable measurements. Additionally, the RI of buffer solutions, such as phosphate-buffered saline (PBS), is unknown. We built a new optical set-up based on the minimum deviation angle to traceably measure the RI of solids and liquids under controlled environmental conditions. We measured the RI of fused silica, 1.470091, and PBS, 1.344599, at 405 nm, 20.00 °C with an expanded uncertainty of 1.4e-6. Our results differ from previously assumed RIs and therefore have practical implications for nanoparticle flow cytometry measurements.
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Martine Kuiper, Richard Koops, Rienk Nieuwland, Ton G. van Leeuwen, and Edwin van der Pol "New method for traceable refractive index determination by measuring the angle of minimum deviation", Proc. SPIE PC12370, Design and Quality for Biomedical Technologies XVI, PC1237002 (6 March 2023); https://doi.org/10.1117/12.2650587
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KEYWORDS
Refractive index
Environmental sensing
Flow cytometry
Liquids
Solids
Nanoparticles
Particles
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