In this project, we aim to build an unlabeled, two-photon imaging-based approach to identify cellular biomarkers in the context of traumatic brain injuries with subcellular imaging resolutions. So far, we have identified NAD(P)H, FAD, LipDH, and Lipofuscin, four main contributors from the brain cells. And we built a math model to quantify the fluorophore contributions directly from the two photon images. Therefore, we can calculate cellular redox state, the lipofuscin level, which is associated with oxidative stress, as well as the mitochondrial organization, cell-matrix interactions using established optical biomarkers in the lab in a more robust way.
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