Multiscale SHG/THG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis tissues and in vitro models (Conference Presentation)

Paul J. Campagnola; Nathan Sandbo; Darian S. James; Emily Shelton; Mark Jones

doi:10.1117/12.2647385

15 March 2023 Multiscale SHG/THG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis tissues and in vitro models (Conference Presentation)

Paul J. Campagnola, Nathan Sandbo, Darian S. James, Emily Shelton, Mark Jones

Author Affiliations +

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840O (2023) https://doi.org/10.1117/12.2647385
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We use SHG microscopy to study collagen alterations in idiopathic pulmonary fibrosis (IPF) in human tissues and in vitro models. Texture analysis successfully classified the fiber morphology in normal and IPF tissues at near 100% accuracy. SHG polarization measurement indicated reduced chirality in the collagen triple helix in IPF in both tissues and spheroid models. We have created a “collagen atlas” in normal and IPF lung using the combination of SHG and THG to image large areas in annotated histology slides. The combined collagen and cellular organization data formed an accurate classification scheme and identified the most sensitive features.

Conference Presentation

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Paul J. Campagnola, Nathan Sandbo, Darian S. James, Emily Shelton, and Mark Jones "Multiscale SHG/THG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis tissues and in vitro models (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840O (15 March 2023); https://doi.org/10.1117/12.2647385

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KEYWORDS

Collagen

Tissues

Pulmonary fibrosis

In vitro testing

Second-harmonic generation

Blood vessels

Microscopy

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