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15 March 2023 SRS, MPF, and SHG integrated multimodal metabolic imaging platform for studying aging and diseases (Conference Presentation)
Lingyan Shi
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Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC1238408 (2023) https://doi.org/10.1117/12.2652704
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We developed a multimodal optical metabolic imaging system that integrates SRS, MPF, and SHG, for studying aging and diseases. We quantitatively measured the metabolic dynamics in cells and animals under various conditions, and further combined deuterium oxide probing with stimulated Raman scattering (DO-SRS) for visualizing newly synthesized protein and lipid molecules, in addition to macromolecules (protein and lipid, NADH and Flavin, collagen) imaged with the label free multimodal microscopy.
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Lingyan Shi "SRS, MPF, and SHG integrated multimodal metabolic imaging platform for studying aging and diseases (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC1238408 (15 March 2023); https://doi.org/10.1117/12.2652704
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KEYWORDS
Second-harmonic generation
Multimodal imaging
Molecules
Multiphoton fluorescence microscopy
Proteins
Raman scattering
Ovary
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