Photoelectron Microscopy Of Biological Surfaces Excitation Source Brightness Requirements

Rudy J. Dam; O.Hayes Griffith

doi:10.1117/12.954794

27 July 1976 Photoelectron Microscopy Of Biological Surfaces Excitation Source Brightness Requirements

Rudy J. Dam, O.Hayes Griffith

Proceedings Volume 0078, Low Light Level Devices for Science and Technolgy; (1976) https://doi.org/10.1117/12.954794
Event: 1976 SPIE/SPSE Technical Symposium East, 1976, Reston, United States

Abstract

Photoelectron microscopy is a surface technique which provides topographical information using the photoelectric effect as a basis for contrast. Progress in the biological applications of this technique is briefly reviewed. Due to relatively low quantum yields, photoemission from biological samples is weak and an image intensifier is used in order to visualize and record the photoelectron image. Currently the limiting magnification is determined by UV power incident on the sample. Power requirements for high-magnification imaging are calculated in terms of microscope, sample, and image intensifier parameters. To approach 40 R resolution, an instrument magnification of 12,000-50,000 is required along with a UV intensity of 0.01 to 10 Watts/cm² depending on the wavelength and sample. For a tightly focused laser source the total power requirement is 1 mWatt or less.

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Rudy J. Dam and O.Hayes Griffith "Photoelectron Microscopy Of Biological Surfaces Excitation Source Brightness Requirements", Proc. SPIE 0078, Low Light Level Devices for Science and Technolgy, (27 July 1976); https://doi.org/10.1117/12.954794

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