Three-Dimensional Analysis of Biological Specimens Utilizing Image Processing Techniques

David A. Agard; John W. Sedat

doi:10.1117/12.959792

21 November 1980 Three-Dimensional Analysis of Biological Specimens Utilizing Image Processing Techniques

David A. Agard, John W. Sedat

Proceedings Volume 0264, Applications of Digital Image Processing to Astronomy; (1980) https://doi.org/10.1117/12.959792
Event: 1981 Los Angeles Technical Symposium, 1980, Los Angeles, United States

Abstract

Examination of biological structures at the level of whole cells presents several major problems that can be solved using two- and three-dimensional reconstruction and image enhancement techniques. Presented here are first steps towards realizing this goal of cellular tomography. General methodologies for extraction of additional information from biological objects using median and bandpass filtration and constrained deconvolution procedures are described. These image processing techniques are ideally suited for studying large non-crystalline objects by either optical or electron microscopy. The ultrastructural architecture of Drosophila sperm heads has been investigated, revealing that these examples of highly-condensed DNA have a defined arrangement based on helical principles.

Citation Download Citation

David A. Agard and John W. Sedat "Three-Dimensional Analysis of Biological Specimens Utilizing Image Processing Techniques", Proc. SPIE 0264, Applications of Digital Image Processing to Astronomy, (21 November 1980); https://doi.org/10.1117/12.959792

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