In Vivo Wavklength Dependent Characteristics Of Hematoporphyrine-Derivative (HpD)

D. Breederveld; G. H.M. Gijsbers; M. J.C. van Gemert; T. A. Boon; J. Langelaar; R. P.H. Rettschnick

doi:10.1117/12.943745

27 March 1985 In Vivo Wavklength Dependent Characteristics Of Hematoporphyrine-Derivative (HpD)

D. Breederveld, G. H.M. Gijsbers, M. J.C. van Gemert, T. A. Boon, J. Langelaar, R. P.H. Rettschnick

Proceedings Volume 0492, 1984 European Conf on Optics, Optical Systems, and Applications; (1985) https://doi.org/10.1117/12.943745
Event: 1984 European Conference on Optics, Optical Systems and Applications, 1984, Amsterdam, Netherlands

Abstract

Fluorescence excitation spectra are recorded from HpD in living RUC-2 tumors implanted in rats. The experiments show that, compared with the excitation spectrum of HpD in solution, the Soret excitation band at 400 nm decreases with respect to the minor excitation bands at longer wavelengths. The excitation efficiencies at 503 nm and 625 nm are comparable with the efficiency at 400 nm. The differences in the in vivo and in vitro excitation spectra are caused by the wavelength dependent penetration depth of the excitation light due to absorption of blood and tissue.

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D. Breederveld, G. H.M. Gijsbers, M. J.C. van Gemert, T. A. Boon, J. Langelaar, and R. P.H. Rettschnick "In Vivo Wavklength Dependent Characteristics Of Hematoporphyrine-Derivative (HpD)", Proc. SPIE 0492, 1984 European Conf on Optics, Optical Systems, and Applications, (27 March 1985); https://doi.org/10.1117/12.943745

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KEYWORDS

Tumors

Blood

Tissues

Luminescence

In vivo imaging

Tissue optics

Absorption

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