Nondestructive Sectioning Of Fixed And Living Specimens Using A Confocal Scanning Laser Fluorescence Microscope: Microtomoscopy

Ernst H.K . Stelzer; Roelof W. Wijnaendts-Van-Resandt

doi:10.1117/12.941509

3 August 1987 Nondestructive Sectioning Of Fixed And Living Specimens Using A Confocal Scanning Laser Fluorescence Microscope: Microtomoscopy

Ernst H.K . Stelzer, Roelof W. Wijnaendts-Van-Resandt

Author Affiliations +

Proceedings Volume 0809, Scanning Imaging Technology; (1987) https://doi.org/10.1117/12.941509
Event: Fourth International Symposium on Optical and Optoelectronic Applied Sciences and Engineering, 1987, The Hague, Netherlands

Abstract

Modern molecular biologists and in particular cell biologists have a large set of experimental tools at their disposal. Immunocytochemistry, fluorescence labels, and microscopy are only subsets of the entire spectrum of methods. Depending on the fields in which biologists work a lot of results are obtained with classical biochemistry, gel electrophoresis and blotting techniques. Gathering morphological data may not be the least important task, but will in many cases be considered only after all other methods have failed. With the advent of video microscopes and the availability of high speed image processing devices, microscopy can also be used for quantitation. Confocal scanning laser fluorescence microscopy (Ft-CSCM) [Cox 1984] is in fact another technique or method that is entering the rapidly developing field of quantitative microscopy. It is therefore very important to understand the physical properties of the CSCLM in detail and to compare a confocal microscope not only with other confocal microscopes, but also with all the other techniques and methods. The confocal microscope has to find its particular application and it should be understood that it will replace neither conventional microscopy, nor video microscopy, nor electron microscopy. It will not be used for every application and every type of investigation. The CSCM has to find its niche in the laboratories and this paper will present two applications in which it proves its usefulness.

Citation Download Citation

Ernst H.K . Stelzer and Roelof W. Wijnaendts-Van-Resandt "Nondestructive Sectioning Of Fixed And Living Specimens Using A Confocal Scanning Laser Fluorescence Microscope: Microtomoscopy", Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); https://doi.org/10.1117/12.941509

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