Quantitative optical imaging of paracetamol-induced metabolism changes in the liver

Xiaowen Liang; Haolu Wang; Xin Liu; Michael Roberts

doi:10.1117/12.2242962

9 December 2016 Quantitative optical imaging of paracetamol-induced metabolism changes in the liver

Xiaowen Liang, Haolu Wang, Xin Liu, Michael Roberts

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 100131H (2016) https://doi.org/10.1117/12.2242962
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

Paracetamol is the most readily available and widely used painkiller. However, its toxicity remains the most common cause of liver injury. The toxicity of paracetamol has been attributing to its toxic metabolite, which depletes cellular glutathione (GSH) stores and reacts within cells to increase oxidative stress, leading to mitochondrial dysfunction and cell necrosis. Multiphoton microscopy (MPM) and fluorescence lifetime imaging (FLIM) can provide quantitative imaging of biological tissues and organs in vivo and allow direct visualization of cellular events, which were used to monitor cellular metabolism in paracetamol-induced toxicity in this study. To better understand mechanisms of paracetamol induced liver injury, the redox ratio of NADH/FAD in liver cells were detected and quantified by MPM imaging to represent the relative rates of glycolysis and oxidative phosphorylation within cells. Compared to normal liver, average fluorescence lifetime of NADH and redox ratio of NADH/FAD in hepatocytes was significantly decreased after paracetamol overdose for 12 and 24 hrs, reflecting impaired metabolic activity. GSH levels of treatment groups were significantly lower than those of normal livers, with gradually decreasing from periportal to centrilobular zonation. This imaging technique has significant implications for investigating metabolic mechanisms of paracetamol toxicity.

Citation Download Citation

Xiaowen Liang, Haolu Wang, Xin Liu, and Michael Roberts "Quantitative optical imaging of paracetamol-induced metabolism changes in the liver", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100131H (9 December 2016); https://doi.org/10.1117/12.2242962

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Liver

Luminescence

Toxicity

Mode conditioning cables

Tissues

Injuries

Fluorescence lifetime imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years