Detection of the multiphoton signals in stained tissue using nonlinear optical microscopy

Yaping Zeng; Jian Xu; Deyong Kang; Jiangbo Lin; Jianxin Chen

doi:10.1117/12.2245825

31 October 2016 Detection of the multiphoton signals in stained tissue using nonlinear optical microscopy

Yaping Zeng, Jian Xu, Deyong Kang, Jiangbo Lin, Jianxin Chen

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 100242L (2016) https://doi.org/10.1117/12.2245825
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging, has become a powerful, important tool for tissue imaging at the molecular level. Recently, MPM is also used to image hematoxylin and eosin (H and E)-stained sections in cancer diagnostics. However, several studies have showed that the MPM images of tissue stained with H and E are significantly different from unstained tissue sections. Our aim was to detect of the multiphoton signals in stained tissue by using MPM. In this paper, MPM was used to image histological sections of esophageal invasive carcinoma tissues stained with H, E, H and E and fresh tissue. To detect of the multiphoton signals in stained tissue, the emission spectroscopic of tissue stained with H, E, H and E were obtained. For comparison, the fresh tissues were also investigated. Our results showed that the tissue stained with H, E, H and E could be detected by their TPEF signals. While the tissue stained with H and fresh tissue could be detected by their TPEF and SHG signals. In this work, we detect of the multiphoton signals in stained tissue. These findings will be useful for choosing suitable staining method so to improve the quality of MPM imaging in the future.

Citation Download Citation

Yaping Zeng, Jian Xu, Deyong Kang, Jiangbo Lin, and Jianxin Chen "Detection of the multiphoton signals in stained tissue using nonlinear optical microscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 100242L (31 October 2016); https://doi.org/10.1117/12.2245825

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