Parameter estimation and analysis model selections in fluorescence correlation spectroscopy

Shiqing Dong; Jie Zhou; Xuemei Ding; Yuhua Wang; Shusen Xie; Hongqin Yang

doi:10.1117/12.2246027

31 October 2016 Parameter estimation and analysis model selections in fluorescence correlation spectroscopy

Shiqing Dong, Jie Zhou, Xuemei Ding, Yuhua Wang, Shusen Xie, Hongqin Yang

Proceedings Volume 10024, Optics in Health Care and Biomedical Optics VII; 1002433 (2016) https://doi.org/10.1117/12.2246027
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

Fluorescence correlation spectroscopy (FCS) is a powerful technique that could provide high temporal resolution and detection for the diffusions of biomolecules at extremely low concentrations. The accuracy of this approach primarily depends on experimental condition requirements and the data analysis model. In this study, we have set up a confocal-based FCS system. And then we used a Rhodamine6G solution to calibrate the system and get the related parameters. An experimental measurement was carried out on one-component solution to evaluate the relationship between a certain number of molecules and concentrations. The results showed FCS system we built was stable and valid. Finally, a two-component solution experiment was carried out to show the importance of analysis model selection. It is a promising method for single molecular diffusion study in living cells.

Citation Download Citation

Shiqing Dong, Jie Zhou, Xuemei Ding, Yuhua Wang, Shusen Xie, and Hongqin Yang "Parameter estimation and analysis model selections in fluorescence correlation spectroscopy", Proc. SPIE 10024, Optics in Health Care and Biomedical Optics VII, 1002433 (31 October 2016); https://doi.org/10.1117/12.2246027

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KEYWORDS

Fluorescence correlation spectroscopy

Diffusion

Luminescence

Molecules

Data modeling

Particles

Chemical analysis

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