Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles

Jun Ando; Takumasa Sekiya; Den Ka; Hiroyuki Yamakoshi; Kosuke Dodo; Mikiko Sodeoka; Satoshi Kawata; Katsumasa Fujita

doi:10.1117/12.2256126

9 February 2017 Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles

Jun Ando, Takumasa Sekiya, Den Ka, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka, Satoshi Kawata, Katsumasa Fujita

Author Affiliations +

Proceedings Volume 10046, Visualizing and Quantifying Drug Distribution in Tissue; 1004606 (2017) https://doi.org/10.1117/12.2256126
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We propose the combination of alkyne-tag and surface-enhanced Raman scattering (SERS) spectroscopy to perform highly-sensitive and selective drug imaging in live cells. Gold nanoparticles are introduced in lysosomes through endocytosis as SERS agents, and the alkyne-tagged drugs are subsequently administered in cells. Raman microscopic observation reveals the arrival of drug in lysosome through enhanced Raman signal of alkyne. Since the peak of alkyne appears in Raman-silent region of biomolecules, selective detection of drugs is possible without background signal of endogenous molecules. From endocytosed gold nanoparticles in living HeLa cells, we observed distinct Raman signal from alkyne-tagged inhibitor of lysosomal enzyme.

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Jun Ando, Takumasa Sekiya, Den Ka, Hiroyuki Yamakoshi, Kosuke Dodo, Mikiko Sodeoka, Satoshi Kawata, and Katsumasa Fujita "Surface-enhanced Raman scattering (SERS) imaging of alkyne-tagged small molecule drug in live cells with endocytosed gold nanoparticles", Proc. SPIE 10046, Visualizing and Quantifying Drug Distribution in Tissue, 1004606 (9 February 2017); https://doi.org/10.1117/12.2256126

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