Real time laser speckle imaging monitoring vascular targeted photodynamic therapy

Ruth Goldschmidt; Kalchenko Vyacheslav; Avigdor Scherz

doi:10.1117/12.2251971

8 February 2017 Real time laser speckle imaging monitoring vascular targeted photodynamic therapy

Ruth Goldschmidt, Kalchenko Vyacheslav, Avigdor Scherz

Proceedings Volume 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI; 100470M (2017) https://doi.org/10.1117/12.2251971
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Laser speckle imaging is a technique that has been developed to non-invasively monitor in vivo blood flow dynamics and vascular structure, at high spatial and temporal resolution. It can record the full-field spatio-temporal characteristics of microcirculation and has therefore, often been used to study the blood flow in tumors after photodynamic therapy (PDT). Yet, there is a paucity of reports on real-time laser speckle imaging (RTLSI) during PDT. Vascular-targeted photodynamic therapy (VTP) with WST11, a water-soluble bacteriochlorophyll derivative, achieves tumor ablation through rapid occlusion of the tumor vasculature followed by a cascade of events that actively kill the tumor cells. WST11-VTP has been already approved for treatment of early/intermediate prostate cancer at a certain drug dose, time and intensity of illumination. Application to other cancers may require different light dosage. However, incomplete vascular occlusion at lower light dose may result in cancer cell survival and tumor relapse while excessive light dose may lead to toxicity of nearby healthy tissues. Here we provide evidence for the feasibility of concomitant RTLSI of the blood flow dynamics in the tumor and surrounding normal tissues during and after WST11-VTP. Fast decrease in the blood flow is followed by partial mild reperfusion and a complete flow arrest within the tumor by the end of illumination. While the primary occlusion of the tumor feeding arteries and draining veins agrees with previous data published by our group, the late effects underscore the significance of light dose control to minimize normal tissue impairment. In conclusion- RTSLI application should allow to optimize VTP efficacy vs toxicity in both the preclinical and clinical arenas.

Conference Presentation

Citation Download Citation

Ruth Goldschmidt, Kalchenko Vyacheslav, and Avigdor Scherz "Real time laser speckle imaging monitoring vascular targeted photodynamic therapy", Proc. SPIE 10047, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVI, 100470M (8 February 2017); https://doi.org/10.1117/12.2251971

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