Design and implementation of a dual-wavelength intrinsic fluorescence camera system

Antonio Ortega-Martinez; Joseph J. Musacchia; Enoch Gutierrez-Herrera; Ying Wang; Walfre Franco

doi:10.1117/12.2253063

14 March 2017 Design and implementation of a dual-wavelength intrinsic fluorescence camera system

Antonio Ortega-Martinez, Joseph J. Musacchia, Enoch Gutierrez-Herrera, Ying Wang, Walfre Franco

Proceedings Volume 10056, Design and Quality for Biomedical Technologies X; 100560U (2017) https://doi.org/10.1117/12.2253063
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Intrinsic UV fluorescence imaging is a technique that permits the observation of spatial differences in emitted fluorescence. It relies on the fluorescence produced by the innate fluorophores in the sample, and thus can be used for marker-less in-vivo assessment of tissue. It has been studied as a tool for the study of the skin, specifically for the classification of lesions, the delimitation of lesion borders and the study of wound healing, among others. In its most basic setup, a sample is excited with a narrow-band UV light source and the resulting fluorescence is imaged with a UV sensitive camera filtered to the emission wavelength of interest. By carefully selecting the excitation/emission pair, we can observe changes in fluorescence associated with physiological processes. One of the main drawbacks of this simple setup is the inability to observe more than a single excitation/emission pair at the same time, as some phenomena are better studied when two or more different pairs are studied simultaneously. In this work, we describe the design and the hardware and software implementation of a dual wavelength portable UV fluorescence imaging system. Its main components are an UV camera, a dual wavelength UV LED illuminator (295 and 345 nm) and two different emission filters (345 and 390 nm) that can be swapped by a mechanical filter wheel. The system is operated using a laptop computer and custom software that performs basic pre-processing to improve the image. The system was designed to allow us to image fluorescent peaks of tryptophan and collagen cross links in order to study wound healing progression.

Conference Presentation

Citation Download Citation

Antonio Ortega-Martinez, Joseph J. Musacchia, Enoch Gutierrez-Herrera, Ying Wang, and Walfre Franco "Design and implementation of a dual-wavelength intrinsic fluorescence camera system", Proc. SPIE 10056, Design and Quality for Biomedical Technologies X, 100560U (14 March 2017); https://doi.org/10.1117/12.2253063

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available