Reliable determination of tissue optical properties from spatially resolved reflectance

Thomas Gladytz; Alexander Hoppe; Kathleen Cantow; Andreas Pohlmann; Bert Flemming; Thoralf Niendorf; Erdmann Seeliger; Dirk Grosenick

doi:10.1117/12.2250552

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14 March 2017 Reliable determination of tissue optical properties from spatially resolved reflectance

Thomas Gladytz, Alexander Hoppe, Kathleen Cantow, Andreas Pohlmann, Bert Flemming, Thoralf Niendorf, Erdmann Seeliger, Dirk Grosenick

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Proceedings Volume 10056, Design and Quality for Biomedical Technologies X; 100560W (2017) https://doi.org/10.1117/12.2250552
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Spatially resolved reflectance is a frequently used technique to derive optical properties and physiological parameters of tissue. We have evaluated the accuracy of this method by investigations on a set of phantoms with known optical properties derived from time-resolved measurements. The recorded profiles of spatially resolved reflectance were analyzed by a Monte Carlo model of photon transport. When we took only the shape of the measured profiles into account, we got only poor estimates of the optical properties. In particular, the absorption was strongly underestimated. The main reason for failing of this approach is that the shape of the measured profiles can be well described by many combinations of absorption and reduced scattering coefficients. The separation between scattering and absorption was strongly improved when the reflectance data were calibrated by using a reference phantom. We applied both the relative and the calibration based analysis method to reflectance data obtained from in vivo investigations on the kidney of rats. Despite the limited number of only 4 detector positions the calibration based analysis method yielded reliable estimates of the tissue optical properties.

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Thomas Gladytz, Alexander Hoppe, Kathleen Cantow, Andreas Pohlmann, Bert Flemming, Thoralf Niendorf, Erdmann Seeliger, and Dirk Grosenick "Reliable determination of tissue optical properties from spatially resolved reflectance", Proc. SPIE 10056, Design and Quality for Biomedical Technologies X, 100560W (14 March 2017); https://doi.org/10.1117/12.2250552

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