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28 February 2017 Microfluidic separation of particles from whole blood using shear induced diffusion
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Extraction of cells of interest directly from human whole blood is in high demand. However, it is extremely challenging due to the excessive cell populations leading to non-Newtonian hemodynamics. Herein, we describe a simple microfluidic approach to take the advantage of the concentrated cells for direct isolation of larger particles spiked in whole blood, which could shift the current paradigm in cell separation from low volume fraction sample to concentrated suspension and from the Newtonian to non-Newtonian fluid. Our device fabricated in Polydimethylsiloxane (PDMS) via standard soft photolithography. With saline solution flowing in the channel center and splitting the blood sample into two side streams, the spiked larger particles rapidly migrate from the whole blood to the saline stream without external force fields. Our experimental results has suggested such intriguing particle translocation is mainly attributed to the shear induced diffusion in concentrated suspensions as well as the viscoelastic property of blood. Inertial force can also contribute to this process. The harmonic interactions among these passive force fields have successfully led to extraction, focusing and separation of 18.7 μm –diameter particles from whole blood with a high efficiency (~89%) and a superb throughput (>106 cells per second), which outperforms existing approaches such as spiral microchannels. Shortly, we have demonstrated a novel simple and effective separation scheme which directly works for complex bodily samples without a hassle of sample preparation. Our approach is very promising as it holds potential to separate cells from whole blood.
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Jian Zhou, Chunglong Tu, Yitao Liang, Bobo Huang, Yifeng Fang, Xiao Liang, Ian Papautsky, and Xuesong Ye "Microfluidic separation of particles from whole blood using shear induced diffusion", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 100610X (28 February 2017);

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