Microfluidic system for in-vitro hypoxia assays

M. Busek; S. Grünzner; T. Steege; C. Steinfelder; F. Schmieder; U. Klotzbach; F. Sonntag

doi:10.1117/12.2253664

28 February 2017 Microfluidic system for in-vitro hypoxia assays

M. Busek, S. Grünzner, T. Steege, C. Steinfelder, F. Schmieder, U. Klotzbach, F. Sonntag

Proceedings Volume 10061, Microfluidics, BioMEMS, and Medical Microsystems XV; 1006110 (2017) https://doi.org/10.1117/12.2253664
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Hereby presented is a microfluidic system, including a micro pump, an oxygenator and a cell culture chamber for perfusion controlled hypoxia assays. It consists of laser-structured polycarbonate (PC) foils and an elastomeric membrane which were joined together using thermal diffusion bonding. The elastomer forms an oxygenator element. The microfluidic system is characterized using non-invasive flow measurement based on micro-Particle-ImageVelocimetry (μPIV) and optical oxygen measurement utilizing the oxygen dependent fluorescence decay. Based on those experimental results and mathematical considerations, the oxygenator and mass transport phenomena within the microfluidic system can be described. This oxygen sensor, the micro pump, a controlling device and the gas mixture at the oxygenator forms a regulatory circuit to adjust the oxygen content in the cell culture chamber and helps to produce well-defined hypoxic conditions for the cells.

Conference Presentation

Citation Download Citation

M. Busek, S. Grünzner, T. Steege, C. Steinfelder, F. Schmieder, U. Klotzbach, and F. Sonntag "Microfluidic system for in-vitro hypoxia assays", Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 1006110 (28 February 2017); https://doi.org/10.1117/12.2253664

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available