In vivo longitudinal visualization of bone marrow engraftment process in mouse calvarium bone marrow with two-photon microscopy (Conference Presentation)

Viet Hoan Le; Seunghun Lee; Ki Hean Kim; Seungwon Lee; Seung-Woo Lee

doi:10.1117/12.2252055

24 April 2017 In vivo longitudinal visualization of bone marrow engraftment process in mouse calvarium bone marrow with two-photon microscopy (Conference Presentation)

Viet Hoan Le, Seunghun Lee, Ki Hean Kim, Seungwon Lee, Seung-Woo Lee

Author Affiliations +

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100690E (2017) https://doi.org/10.1117/12.2252055
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Bone marrow transplantation became the standard choice for treatment of many leukemias, tumors and metabolic diseases. Understanding the dynamic behavior of bone marrow niches, especially in case of bone marrow transplantation is critical to improve the efficiency of the treatment. Intravital microscopy was demonstrated to be a powerful tool to study physiological structure of bone marrow niche. However, current method of intravital microscopy has difficulty in longitudinal monitoring the same bone marrow niche site due to the invasion of the prior-imaging surgery. In this study, we introduce a method to improve the bone marrow niche imaging process and enable the longitudinal imaging of murine calvarium bone marrow. Mouse model for calvarium bone marrow imaging was made by attaching cover glass window to the calvarium bone. Longitudinal imaging of whole bone marrow engraftment process was carried out to demonstrate the advantage of our mouse model. Qualitative and quantitative analysis were also executed on the image data. The result provided a dynamic and full visualization of the bone marrow engraftment process. The study was expected to provide helpful tool for bone marrow studies and useful information for bone marrow transplantation in future.

Conference Presentation

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Viet Hoan Le, Seunghun Lee, Ki Hean Kim, Seungwon Lee, and Seung-Woo Lee "In vivo longitudinal visualization of bone marrow engraftment process in mouse calvarium bone marrow with two-photon microscopy (Conference Presentation)", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690E (24 April 2017); https://doi.org/10.1117/12.2252055

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KEYWORDS

Bone

Transplantation

Visualization

In vivo imaging

Microscopy

Mouse models

Two photon excitation microscopy

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