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21 February 2017 New approaches to intracellular drug imaging by stimulated Raman scattering microscopy
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A range of proposed alkyne Raman tags are examined in-silico for activity and then synthesised generating a library of analogues of the natural product, anisomycin. We report the use of bisaryl butadiyne-anisomycin (BADY-anisomycin) in intracellular SRS microscopy studies of uptake and localisation within live and fixed cells. Following rational design and synthesis, BADY-anisomycin was shown to produce an intense Raman band at 2219 cm-1, that is centrally located within the cellular silent region and is approximately 60 times more Raman active than the corresponding propargylanisomycin. Finally, we demonstrate two-colour imaging utilising EdU, an alkyne-containing proliferation probe and BADY-anisomycin.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
M. Lee, W. J. Tipping, A. Serrels, A. N. Hulme, and V. G. Brunton "New approaches to intracellular drug imaging by stimulated Raman scattering microscopy", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100690T (21 February 2017);

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