Online-FLIM at 10 images per second

Wolfgang Becker; Stefan Smietana

doi:10.1117/12.2254725

21 February 2017 Online-FLIM at 10 images per second

Wolfgang Becker, Stefan Smietana

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 1006916 (2017) https://doi.org/10.1117/12.2254725
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We report on a technique that records and displays fluorescence lifetime images at a rate of 10 images per second. Data acquisition is based on multi-dimensional TCSPC in combination with confocal or two-photon laser scanning. The image are calculated from the TCSPC FLIM data via the first moment of the decay data in the pixels of the images. The first-moment technique combines near-ideal photon efficiency with calculation times shorter than the frame times of the commonly used galvanometer scanners. The image rate is thus not slowed down by the lifetime calculation process. Potential applications are clinical FLIM, where suspicious areas can be identified for subsequent high-accuracy imaging, and standard FLIM microscopy, where the technique it helps the user select interesting cells in a large field of view for detailed analysis.

Conference Presentation

Citation Download Citation

Wolfgang Becker and Stefan Smietana "Online-FLIM at 10 images per second", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006916 (21 February 2017); https://doi.org/10.1117/12.2254725

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