Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy

Junwei Min; Baoli Yao; Steffi Ketelhut; Björn Kemper

doi:10.1117/12.2252883

17 February 2017 Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy

Junwei Min, Baoli Yao, Steffi Ketelhut, Björn Kemper

Author Affiliations +

Proceedings Volume 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV; 100700A (2017) https://doi.org/10.1117/12.2252883
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The modular combination of optical microscopes with digital holographic microscopy (DHM) has been proven to be a powerful tool for quantitative live cell imaging. The introduction of condenser and different microscope objectives (MO) simplifies the usage of the technique and makes it easier to measure different kinds of specimens with different magnifications. However, the high flexibility of illumination and imaging also causes variable phase aberrations that need to be eliminated for high resolution quantitative phase imaging. The existent phase aberrations compensation methods either require add additional elements into the reference arm or need specimen free reference areas or separate reference holograms to build up suitable digital phase masks. These inherent requirements make them unpractical for usage with highly variable illumination and imaging systems and prevent on-line monitoring of living cells. In this paper, we present a simple numerical method for phase aberration compensation based on the analysis of holograms in spatial frequency domain with capabilities for on-line quantitative phase imaging. From a single shot off-axis hologram, the whole phase aberration can be eliminated automatically without numerical fitting or pre-knowledge of the setup. The capabilities and robustness for quantitative phase imaging of living cancer cells are demonstrated.

Citation Download Citation

Junwei Min, Baoli Yao, Steffi Ketelhut, and Björn Kemper "Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy", Proc. SPIE 10070, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIV, 100700A (17 February 2017); https://doi.org/10.1117/12.2252883

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Digital holography

Holograms

Phase imaging

Holography

Microscopy

Digital recording

Imaging systems

Show All Keywords

Keywords/Phrases

Search In:

Publication Years