Chemotaxis of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy

Björn Kemper; Jürgen Schnekenburger; Steffi Ketelhut

doi:10.1117/12.2253091

21 February 2017 Chemotaxis of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy

Björn Kemper, Jürgen Schnekenburger, Steffi Ketelhut

Author Affiliations +

Proceedings Volume 10074, Quantitative Phase Imaging III; 1007412 (2017) https://doi.org/10.1117/12.2253091
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We investigated the capabilities of digital holographic microscopy (DHM) for label-free quantification of the response of living single cells to chemical stimuli in 3D assays. Fibro sarcoma cells were observed in a collagen matrix inside 3D chemotaxis chambers with a Mach-Zehnder interferometer-based DHM setup. From the obtained series of quantitative phase images, the migration trajectories of single cells were retrieved by automated cell tracking and subsequently analyzed for maximum migration distance and motility. Our results demonstrate DHM as a highly reliable and efficient tool for label-free quantification of chemotaxis in 2D and 3D environments.

Citation Download Citation

Björn Kemper, Jürgen Schnekenburger, and Steffi Ketelhut "Chemotaxis of cancer cells in three-dimensional environment monitored label-free by quantitative phase digital holographic microscopy", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007412 (21 February 2017); https://doi.org/10.1117/12.2253091

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