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24 April 2017 Three-dimensional refractive index and fluorescence tomography using structured illumination (Conference Presentation)
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Proceedings Volume 10074, Quantitative Phase Imaging III; 1007415 (2017) https://doi.org/10.1117/12.2255702
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
Optical diffraction tomography (ODT) has been an emerging optical technique for label-free imaging of three-dimensional (3-D) refractive index (RI) distribution of biological samples. ODT employs interferometric microscopy for measuring multiple holograms of samples with various incident angles, from which the Fourier diffraction theorem reconstructs the 3-D RI distribution of samples from retrieved complex optical fields. Since the RI value is linearly proportional to the protein concentration of biological samples where the proportional coefficient is called as refractive index increment (RII), reconstructed 3-D RI tomograms provide precise structural and biochemical information of individual biological samples. Because most proteins have similar RII value, however, ODT has limited molecular specificity, especially for imaging eukaryotic cells having various types of proteins and subcellular organelles. Here, we present an ODT system combined with structured illumination microscopy which can measure the 3-D RI distribution of biological samples as well as 3-D super-resolution fluorescent images in the same optical setup. A digital micromirror device (DMD) controls the incident angle of the illumination beam for tomogram reconstruction, and the same DMD modulates the structured illumination pattern of the excitation beam for super-resolution fluorescent imaging. We first validate the proposed method for simultaneous optical diffraction tomographic imaging and super-resolution fluorescent imaging of fluorescent beads. The proposed method is also exploited for various biological samples.
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© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
GwangSik Park, SeungWoo Shin, Kyoohyun Kim, and YongKeun Park "Three-dimensional refractive index and fluorescence tomography using structured illumination (Conference Presentation)", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007415 (24 April 2017); https://doi.org/10.1117/12.2255702
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