Opportunities of QPI in the epigenetic diagnostics and assessment of therapeutic efficacy

Irina Vasilenko; Vladislav Metelin; Aleksandr Kuznetsov; Vladimir Belyakov; Tatiana Yakushina

doi:10.1117/12.2253033

21 February 2017 Opportunities of QPI in the epigenetic diagnostics and assessment of therapeutic efficacy

Irina Vasilenko, Vladislav Metelin, Aleksandr Kuznetsov, Vladimir Belyakov, Tatiana Yakushina

Proceedings Volume 10074, Quantitative Phase Imaging III; 1007417 (2017) https://doi.org/10.1117/12.2253033
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Quantitative phase imaging (QPI) can be considered as a potential tool to extract important information on the refractive index of the cellular and subcellular structures. Interphase chromatin is an original biosensor, a detector of early changes in morphofunctional cell condition. The authors presented a technology of densitometric segmentation based on the quantitative phase microscopy and computed analysis of the changes in the optic density of interphase chromatin as a biosensor. The purpose of this work to evaluate the possibility of quantitative phase imaging for visualizing the nuclear proteins conformation and chromatin decondensation degree. Modification of chromatin structure, compactness of its package, and so on, are indicative of cell condition alteration and may be projected on the organism as a whole not only for the early preclinical diagnostics but also for assessment of prognosis in crisis conditions.

Conference Presentation

Citation Download Citation

Irina Vasilenko, Vladislav Metelin, Aleksandr Kuznetsov, Vladimir Belyakov, and Tatiana Yakushina "Opportunities of QPI in the epigenetic diagnostics and assessment of therapeutic efficacy", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007417 (21 February 2017); https://doi.org/10.1117/12.2253033

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