Reconstruction method for extended depth-of-field limited-angle tomography

Wojciech Krauze; Arkadiusz Kuś; Ewa Skrzypek M.D.; Małgorzata Kujawińska

doi:10.1117/12.2251842

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21 February 2017 Reconstruction method for extended depth-of-field limited-angle tomography

Wojciech Krauze, Arkadiusz Kuś, Ewa Skrzypek M.D., Małgorzata Kujawińska

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Proceedings Volume 10074, Quantitative Phase Imaging III; 100741D (2017) https://doi.org/10.1117/12.2251842
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Limited-angle optical diffraction tomography (LAODT) is a powerful tool for measuring 3D refractive index distribution in biological microsamples. However, when thick objects are measured, reconstructions are erroneous due to diffraction errors even in the case when tomographic reconstruction algorithms take into account this phenomenon. We propose a hardware-based solution which allows to change a focal plane position with a liquid tunable lens in LAODT system. For each illumination angle, projections with different focal plane positions are recorded, and thus diffraction errors in the neighborhood of these planes are minimized. In this paper, we describe a method for processing data from a varifocal tomography setup that utilizes a Generalized Total Variation Iterative Constraint algorithm.

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Wojciech Krauze, Arkadiusz Kuś, Ewa Skrzypek M.D., and Małgorzata Kujawińska "Reconstruction method for extended depth-of-field limited-angle tomography", Proc. SPIE 10074, Quantitative Phase Imaging III, 100741D (21 February 2017); https://doi.org/10.1117/12.2251842

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