The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging

Won Sang Hwang; Dong Eun Kim; Jun Woo Kim; Youn Young Ji; Byung Hwy So; Dug Young Kim

doi:10.1117/12.2250730

22 February 2017 The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging

Won Sang Hwang, Dong Eun Kim, Jun Woo Kim, Youn Young Ji, Byung Hwy So, Dug Young Kim

Author Affiliations +

Proceedings Volume 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II; 100761H (2017) https://doi.org/10.1117/12.2250730
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We have simulated the effects of the number of bits and the sampling rate of a digitizer on the performance of lifetime measurements. We found that the number of bits of a digitizer is important to obtain certain accuracy in lifetime measurement. There exists a certain critical sampling frequency of a digitizer required to separate a certain lifetime differences in a double exponentially decaying intensity profile. We did these simulations by using Monte Carlo simulations with least-square curve fitting algorithms.

Citation Download Citation

Won Sang Hwang, Dong Eun Kim, Jun Woo Kim, Youn Young Ji, Byung Hwy So, and Dug Young Kim "The effect of bit number and sampling rate of a digitizer on least-square multi exponential decay analysis in fluorescence lifetime imaging", Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 100761H (22 February 2017); https://doi.org/10.1117/12.2250730

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