Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

Surekha Barkur; Aseefhali Bankapur; Santhosh Chidangil

doi:10.1117/12.2248461

11 November 2016 Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis

Surekha Barkur, Aseefhali Bankapur, Santhosh Chidangil

Proceedings Volume 10150, Second International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2016); 1015016 (2016) https://doi.org/10.1117/12.2248461
Event: Second International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2016), 2016, Bali, Indonesia

Abstract

Raman tweezers spectroscopy study of effect of free radicals was carried out on erythrocytes. We prepared hydroxyl radicals using Fenton reaction (which yields hydroxyl radicals). Raman spectra were acquired from single, trapped erythrocytes after supplementing with these free radicals. The changes in the Raman bands such as 1211 cm^-1, 1224 cm^-1, 1375 cm^-1 indicate deoxygenation of red blood cells (RBCs). Our study shows that free radicals can induce oxidative stress on erythrocytes. The changes in the Raman spectra as well as shape of erythrocytes indicate that oxidative stress can trigger eryptosis in erythrocytes.

Citation Download Citation

Surekha Barkur, Aseefhali Bankapur, and Santhosh Chidangil "Raman tweezers spectroscopy study of free radical induced oxidative stress leading to eryptosis", Proc. SPIE 10150, Second International Seminar on Photonics, Optics, and Its Applications (ISPhOA 2016), 1015016 (11 November 2016); https://doi.org/10.1117/12.2248461

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available