Absorption contrast imaging beyond the diffraction limit with electron-beam excitation assisted optical microscope

Wataru Inami; Masahiro Fukuta; Yoshimasa Kawata; Susumu Terakawa

doi:10.1117/12.2269437

18 April 2017 Absorption contrast imaging beyond the diffraction limit with electron-beam excitation assisted optical microscope

Wataru Inami, Masahiro Fukuta, Yoshimasa Kawata, Susumu Terakawa

Author Affiliations +

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510G (2017) https://doi.org/10.1117/12.2269437
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

We demonstrated that the high spatial resolution absorption contrast imaging of the crystal of vitamin B9 having absorption at UV wavelengths. The absorption wavelength matches with the wavelength of the emission of the fluorescent thin film of an electron-beam excitation assisted (EXA) optical microscope. The fine crystal structure was imaged beyond the optical diffraction limit. The image contrast corresponded with the thickness of the crystal. The illumination light is absorbed with the vitamin B9 crystal and the intensity of the transmitted light depends on the thickness of the vitamin B9 crystal. The EXA optical microscope is useful for analysis of growth of a crystal, bioimaging, and so on.

Citation Download Citation

Wataru Inami, Masahiro Fukuta, Yoshimasa Kawata, and Susumu Terakawa "Absorption contrast imaging beyond the diffraction limit with electron-beam excitation assisted optical microscope", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510G (18 April 2017); https://doi.org/10.1117/12.2269437

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