Adaptive optical imaging through complex living plant cells

Yosuke Tamada; Yutaka Hayano; Takashi Murata; Shin Oya; Yusuke Honma; Minoru Kanazawa; Noriaki Miura; Mitsuyasu Hasebe; Yasuhiro Kamei; Masayuki Hattori

doi:10.1117/12.2275419

18 April 2017 Adaptive optical imaging through complex living plant cells

Yosuke Tamada, Yutaka Hayano, Takashi Murata, Shin Oya, Yusuke Honma, Minoru Kanazawa, Noriaki Miura, Mitsuyasu Hasebe, Yasuhiro Kamei, Masayuki Hattori

Author Affiliations +

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510K (2017) https://doi.org/10.1117/12.2275419
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Live-cell imaging using fluorescent molecules is now essential for biological researches. However, images of living cells are accompanied with blur, which becomes stronger according to the depth inside the cells and tissues. This image blur is caused by the disturbance on light that goes through optically inhomogeneous living cells and tissues. Here, we show adaptive optics (AO) imaging of living plant cells. AO has been developed in astronomy to correct the disturbance on light caused by atmospheric turbulence. We developed AO microscope effective for the observation of living plant cells with strong disturbance by chloroplasts, and successfully obtained clear images inside plant cells.

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Yosuke Tamada, Yutaka Hayano, Takashi Murata, Shin Oya, Yusuke Honma, Minoru Kanazawa, Noriaki Miura, Mitsuyasu Hasebe, Yasuhiro Kamei, and Masayuki Hattori "Adaptive optical imaging through complex living plant cells", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510K (18 April 2017); https://doi.org/10.1117/12.2275419

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