Requirement of spatiotemporal resolution for imaging intracellular temperature distribution

Noriko Hiroi; Ryuichi Tanimoto; Kaito Ii; Mitsunori Ozeki; Kota Mashimo; Akira Funahashi

doi:10.1117/12.2275012

18 April 2017 Requirement of spatiotemporal resolution for imaging intracellular temperature distribution

Noriko Hiroi, Ryuichi Tanimoto, Kaito Ii, Mitsunori Ozeki, Kota Mashimo, Akira Funahashi

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102510Q (2017) https://doi.org/10.1117/12.2275012
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

Intracellular temperature distribution is an emerging target in biology nowadays. Because thermal diffusion is rapid dynamics in comparison with molecular diffusion, we need a spatiotemporally high-resolution imaging technology to catch this phenomenon. We demonstrate that time-lapse imaging which consists of single-shot 3D volume images acquired at high-speed camera rate is desired for the imaging of intracellular thermal diffusion based on the simulation results of thermal diffusion from a nucleus to cytosol.

Citation Download Citation

Noriko Hiroi, Ryuichi Tanimoto, Kaito Ii, Mitsunori Ozeki, Kota Mashimo, and Akira Funahashi "Requirement of spatiotemporal resolution for imaging intracellular temperature distribution", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102510Q (18 April 2017); https://doi.org/10.1117/12.2275012

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