Determination of three-dimensional molecular orientation of type-I collagen by circularly-polarized second harmonic generation imaging

Guan-Yu Zhuo; Wei-Han Hung; Fu-Jen Kao

doi:10.1117/12.2269427

18 April 2017 Determination of three-dimensional molecular orientation of type-I collagen by circularly-polarized second harmonic generation imaging

Guan-Yu Zhuo, Wei-Han Hung, Fu-Jen Kao

Author Affiliations +

Proceedings Volume 10251, Biomedical Imaging and Sensing Conference; 102511K (2017) https://doi.org/10.1117/12.2269427
Event: SPIE Technologies and Applications of Structured Light, 2017, Yokohama, Japan

Abstract

The content of collagen is up to 30% existing in mammals. It supports the main component of connective tissues such as skin, ligament, and cartilage. Among various types of collagen, type-I collagen is of the most abundance and has been broadly studied due to the importance in bioscience. Second harmonic generation (SHG) microscopy is an effective tool used to study the collagen organization without labeling. In this study, we used circular polarization instead of linear polarization to retrieve three-dimensional (3D) molecular orientation of type-I collagen with only two cross polarized SHG images without acquiring an image stack of varying polarization.

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Guan-Yu Zhuo, Wei-Han Hung, and Fu-Jen Kao "Determination of three-dimensional molecular orientation of type-I collagen by circularly-polarized second harmonic generation imaging", Proc. SPIE 10251, Biomedical Imaging and Sensing Conference, 102511K (18 April 2017); https://doi.org/10.1117/12.2269427

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KEYWORDS

Collagen

Second-harmonic generation

3D image processing

Polarization

Harmonic generation

Microscopy

Tissues

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