Luminescence resonance energy transfer (LRET) aptasensor for ochratoxin A detection using upconversion nanoparticles

Eun-Jung Jo; Ju-Young Byun; Hyoyoung Mun; Min-Gon Kim

doi:10.1117/12.2267206

12 July 2017 Luminescence resonance energy transfer (LRET) aptasensor for ochratoxin A detection using upconversion nanoparticles

Eun-Jung Jo, Ju-Young Byun, Hyoyoung Mun, Min-Gon Kim

Author Affiliations +

Proceedings Volume 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017; 1032404 (2017) https://doi.org/10.1117/12.2267206
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 2017, Jeju, Korea, Republic of

Abstract

We report an aptasensor for homogeneous ochratoxin A (OTA) detection based on luminescence resonance energy transfer (LRET). This system uses upconversion nanoparticles (UCNPs), such as NaYF₄:Yb³⁺, Er ³⁺, as the donor. The aptamer includes the optimum-length linker (5-mer-length DNA) and OTA-specific aptamer sequences. Black hole quencher 1 (BHQ1), as the acceptor, was modified at the 3' end of the aptamer sequence. BHQ1 plays as a quencher in LRET aptasensor and shows absorption at 543 nm, which overlaps with well the emission of the UCNPs. When OTA is added, the BHQ1-labeled OTA aptamer was folded due to the formation of the G-quadruplex-OTA complex, which induced the BHQ1 close to the UCNPs. Consequently, resonance energy transfer between UCNPs (donor) and BHQ1 (acceptor) enables quenching of upconversion luminescence signals under laser irradiation of 980 nm. Our results showed that the LRET-based aptasensor allows specific OTA analysis with a limit of detection of 0.03 ng/mL. These results demonstrated that the OTA in diverse foods can be detected specifically and sensitively in a homogeneous manner.

Citation Download Citation

Eun-Jung Jo, Ju-Young Byun, Hyoyoung Mun, and Min-Gon Kim "Luminescence resonance energy transfer (LRET) aptasensor for ochratoxin A detection using upconversion nanoparticles", Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 1032404 (12 July 2017); https://doi.org/10.1117/12.2267206

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