Scanless nonlinear optical microscope for image reconstruction and space-time correlation analysis

N. G. Ceffa; F. Radaelli; P. Pozzi; M. Collini; L. Sironi; L. D'alfonso; G. Chirico

doi:10.1117/12.2271832

26 June 2017 Scanless nonlinear optical microscope for image reconstruction and space-time correlation analysis

N. G. Ceffa, F. Radaelli, P. Pozzi, M. Collini, L. Sironi, L. D'alfonso, G. Chirico

Proceedings Volume 10333, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III; 103330E (2017) https://doi.org/10.1117/12.2271832
Event: SPIE Optical Metrology, 2017, Munich, Germany

Abstract

Optical Microscopy has been applied to life science from its birth and reached widespread application due to its major advantages: limited perturbation of the biological tissue and the easy accessibility of the light sources. However, as the spatial and time resolution requirements and the time stability of the microscopes increase, researchers are struggling against some of its limitations: limited transparency and the refractivity of the living tissue to light and the field perturbations induced by the path in the tissue. We have developed a compact stand-alone, completely scan-less, optical setup that allows to acquire non-linear excitation images and to measure the sample dynamics simultaneously on an ensemble of arbitrary chosen regions of interests. The image is obtained by shining a square array of spots on the sample obtained by a spatial light modulator and by shifting it (10 ms refresh time) on the sample. The final image is computed from the superposition of (100-1000) images. Filtering procedures can be applied to the raw images of the excitation array before building the image. We discuss results that show how this setup can be used for the correction of wave front aberrations induced by turbid samples (such as living tissues) and for the computation of space-time cross-correlations in complex networks.

Citation Download Citation

N. G. Ceffa, F. Radaelli, P. Pozzi, M. Collini, L. Sironi, L. D'alfonso, and G. Chirico "Scanless nonlinear optical microscope for image reconstruction and space-time correlation analysis", Proc. SPIE 10333, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III, 103330E (26 June 2017); https://doi.org/10.1117/12.2271832

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