Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy

Jongho Baek; Jieqiong Lou; Simao Coelho; Yean Jin Lim; Silvia Seidlitz; Philip R. Nicovich; Christian Wunder; Ludger Johannes; Katharina Gaus

doi:10.1117/12.2275706

29 April 2017 Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy

Jongho Baek, Jieqiong Lou, Simao Coelho, Yean Jin Lim, Silvia Seidlitz, Philip R. Nicovich, Christian Wunder, Ludger Johannes, Katharina Gaus

Author Affiliations +

Proceedings Volume 10340, International Conference on Biophotonics V; 103400J (2017) https://doi.org/10.1117/12.2275706
Event: International Conference on Biophotonics V, 2017, Perth, Australia

Abstract

Lattice light-sheet (LLS) microscopy provides ultrathin light sheets of a two-dimensional optical lattice that allows us imaging three-dimensional (3D) objects for hundreds of time points at sub-second intervals and at or below the diffraction limit. Galectin-3 (Gal3), a carbohydrate-binding protein, triggers glycosphingolipid (GSL)-dependent biogenesis of morphologically distinct endocytic vesicles that are cargo specific and clathrin independent. In this study, we apply LLS microscopy to study the dynamics of Gal3 dependent endocytosis in live T cells. This will allow us to observe Gal3-mediated endocytosis at high temporal and excellent 3D spatial resolution, which may shed light on our understanding of the mechanism and physiological function of Gal3-induced endocytosis.

Citation Download Citation

Jongho Baek, Jieqiong Lou, Simao Coelho, Yean Jin Lim, Silvia Seidlitz, Philip R. Nicovich, Christian Wunder, Ludger Johannes, and Katharina Gaus "Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy", Proc. SPIE 10340, International Conference on Biophotonics V, 103400J (29 April 2017); https://doi.org/10.1117/12.2275706

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available