Development of dielectrophoresis MEMS device for PC12 cell patterning to elucidate nerve-network generation

Eiji Nakamachi; Hirotaka Koga; Yusuke Morita; Koji Yamamoto; Hidetoshi Sakamoto

doi:10.1117/12.2281491

2 January 2018 Development of dielectrophoresis MEMS device for PC12 cell patterning to elucidate nerve-network generation

Eiji Nakamachi, Hirotaka Koga, Yusuke Morita, Koji Yamamoto, Hidetoshi Sakamoto

Author Affiliations +

Proceedings Volume 10456, Nanophotonics Australasia 2017; 104562R (2018) https://doi.org/10.1117/12.2281491
Event: SPIE Nanophotonics Australasia, 2017, Melbourne, Australia

Abstract

We developed a PC12 cell trapping and patterning device by combining the dielectrophoresis (DEP) methodology and the micro electro mechanical systems (MEMS) technology for time-lapse observation of morphological change of nerve network to elucidate the generation mechanism of neural network. We succeeded a neural network generation, which consisted of cell body, axon and dendrites by using tetragonal and hexagonal cell patterning. Further, the time laps observations was carried out to evaluate the axonal extension rate. The axon extended in the channel and reached to the target cell body. We found that the shorter the PC12 cell distance, the less the axonal connection time in both tetragonal and hexagonal structures. After 48 hours culture, a maximum success rate of network formation was 85% in the case of 40 μm distance tetragonal structure.

Citation Download Citation

Eiji Nakamachi, Hirotaka Koga, Yusuke Morita, Koji Yamamoto, and Hidetoshi Sakamoto "Development of dielectrophoresis MEMS device for PC12 cell patterning to elucidate nerve-network generation", Proc. SPIE 10456, Nanophotonics Australasia 2017, 104562R (2 January 2018); https://doi.org/10.1117/12.2281491

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