Feasibility study of Raman spectroscopy for investigating the mouse retina in vivo

Suman K. Manna; Marcos A. S. de Oliveira; Pengfei Zhang; Ratheesh K. Meleppat; Che-Wei Chang; Edward N. Pugh Jr.; James W. Chan; Robert J. Zawadzki

doi:10.1117/12.2288790

19 March 2018 Feasibility study of Raman spectroscopy for investigating the mouse retina in vivo

Suman K. Manna, Marcos A. S. de Oliveira, Pengfei Zhang, Ratheesh K. Meleppat, Che-Wei Chang, Edward N. Pugh Jr., James W. Chan, Robert J. Zawadzki

Author Affiliations +

Proceedings Volume 10474, Ophthalmic Technologies XXVIII; 104741J (2018) https://doi.org/10.1117/12.2288790
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The use of Raman spectroscopy in biochemistry has been very successful, particularly because of its ability to identify elementary chemical species. However, application of this spectroscopic technique for in vivo assessment is often limited by autofluorescence, which make detection of Raman signatures difficult. The mouse eye has been used as an optical testbed for investigation of a variety of disease models and therapeutic pathways. Implementation of in vivo Raman spectroscopy in mice retina would be valuable but needs to be examined in context of the intrinsic auto-fluorescence artifact and potential light damage if high probing beam powers were used. To evaluate feasibility, a Raman system was built on a custom SLO/OCT platform allowing mouse positioning and morphological data acquisition along with the Raman signal from a desired retinal eccentricity. The performance of the Raman system was first assessed with a model eye consisting of polystyrene in the image plane (retina), using excitation wavelengths of 488 nm, 561 nm, and 785 nm to determine whether auto-fluorescence would be reduced at longer wavelengths. To improve the SNR, the combined system is featured with the optical compatibility for these three excitations such that their corresponding spectra from a typical region of interest can be acquired consecutively during single imaging run. Our results include emission spectra acquired over 10 s with excitation energy less than 160 J^.s^-1.m^-2 for all wavelengths and corresponding retinal morphology for different mouse strains including WT, BALB/c and ABCA4^-/-.

Citation Download Citation

Suman K. Manna, Marcos A. S. de Oliveira, Pengfei Zhang, Ratheesh K. Meleppat, Che-Wei Chang, Edward N. Pugh Jr., James W. Chan, and Robert J. Zawadzki "Feasibility study of Raman spectroscopy for investigating the mouse retina in vivo", Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 104741J (19 March 2018); https://doi.org/10.1117/12.2288790

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Raman spectroscopy

Eye

Retina

In vivo imaging

Imaging systems

Scanning laser ophthalmoscopy

Luminescence

Show All Keywords

Keywords/Phrases

Search In:

Publication Years