Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack

Pu-Ting Dong; Haroon Mohammad; Jie Hui; Xiaoyu Wang; Junjie Li; Lijia Liang; Mohamed N. Seleem; Ji-Xin Cheng

doi:10.1117/12.2291248

8 February 2018 Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack

Pu-Ting Dong, Haroon Mohammad, Jie Hui, Xiaoyu Wang, Junjie Li, Lijia Liang, Mohamed N. Seleem, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume 10479, Light-Based Diagnosis and Treatment of Infectious Diseases; 104790R (2018) https://doi.org/10.1117/12.2291248
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Given that the dearth of new antibiotic development loads an existential burden on successful infectious disease therapy, health organizations are calling for alternative approaches to combat methicillin-resistant Staphylococcus aureus (MRSA) infections. Here, we report a drug-free photonic approach to eliminate MRSA through photobleaching of staphyloxanthin, an indispensable membrane-bound antioxidant of S. aureus. The photobleaching process, uncovered through a transient absorption imaging study and quantitated by absorption spectroscopy and mass spectrometry, decomposes staphyloxanthin, and sensitizes MRSA to reactive oxygen species attack. Consequently, staphyloxanthin bleaching by low-level blue light eradicates MRSA synergistically with external or internal reactive oxygen species. The effectiveness of this synergistic therapy is validated in MRSA culture, MRSAinfected macrophage cells. Collectively, these findings highlight broad applications of staphyloxanthin photobleaching for treatment of MRSA infections.

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Citation Download Citation

Pu-Ting Dong, Haroon Mohammad, Jie Hui, Xiaoyu Wang, Junjie Li, Lijia Liang, Mohamed N. Seleem, and Ji-Xin Cheng "Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack", Proc. SPIE 10479, Light-Based Diagnosis and Treatment of Infectious Diseases, 104790R (8 February 2018); https://doi.org/10.1117/12.2291248

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