In vivo imaging of cerebral hemodynamics and tissue scattering in rat brain using a surgical microscope camera system

Izumi Nishidate; Takuya Kanie; Afrina Mustari; Satoko Kawauchi; Shunichi Sato; Manabu Sato; Yasuaki Kokubo

doi:10.1117/12.2290488

8 February 2018 In vivo imaging of cerebral hemodynamics and tissue scattering in rat brain using a surgical microscope camera system

Izumi Nishidate, Takuya Kanie, Afrina Mustari, Satoko Kawauchi, Shunichi Sato, Manabu Sato, Yasuaki Kokubo

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Proceedings Volume 10480, Clinical and Translational Neurophotonics 2018; 104800M (2018) https://doi.org/10.1117/12.2290488
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We investigated a rapid imaging method to monitor the spatial distribution of total hemoglobin concentration (CHbT), the tissue oxygen saturation (StO2), and the scattering power b in the expression of musp=a(lambda)^-b as the scattering parameters in cerebral cortex using a digital red-green-blue camera. In the method, Monte Carlo simulation (MCS) for light transport in brain tissue is used to specify a relation among the RGB-values and the concentration of oxygenated hemoglobin (C_HbO), that of deoxygenated hemoglobin (CHbR), and the scattering power b. In the present study, we performed sequential recordings of RGB images of in vivo exposed brain of rats while changing the fraction of inspired oxygen (FiO₂), using a surgical microscope camera system. The time courses of C_HbO, C_HbR, C_HbT, and StO₂ indicated the well-known physiological responses in cerebral cortex. On the other hand, a fast decrease in the scattering power b was observed immediately after the respiratory arrest, which is similar to the negative deflection of the extracellular DC potential so-called anoxic depolarization. It is said that the DC shift coincident with a rise in extracellular potassium and can evoke cell deformation generated by water movement between intracellular and extracellular compartments, and hence the light scattering by tissue. Therefore, the decrease in the scattering power b after the respiratory arrest is indicative of changes in light scattering by tissue. The results in this study indicate potential of the method to evaluate the pathophysiological conditions and loss of tissue viability in brain tissue.

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Izumi Nishidate, Takuya Kanie, Afrina Mustari, Satoko Kawauchi, Shunichi Sato, Manabu Sato, and Yasuaki Kokubo "In vivo imaging of cerebral hemodynamics and tissue scattering in rat brain using a surgical microscope camera system", Proc. SPIE 10480, Clinical and Translational Neurophotonics 2018, 104800M (8 February 2018); https://doi.org/10.1117/12.2290488

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KEYWORDS

Scattering

Tissues

Brain

Light scattering

Oxygen

Hemodynamics

RGB color model

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