UbasM: a simple, rapid, efficient balanced optical clearing method for brain imaging

Lingling Chen; Guiye Li; Yingchao Li; Lina Liu; Ang Liu; Shuangchen Ruan

doi:10.1117/12.2283223

12 February 2018 UbasM: a simple, rapid, efficient balanced optical clearing method for brain imaging

Lingling Chen, Guiye Li, Yingchao Li, Lina Liu, Ang Liu, Shuangchen Ruan

Proceedings Volume 10481, Neural Imaging and Sensing 2018; 104810W (2018) https://doi.org/10.1117/12.2283223
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Optical clearing methods are highly in demand in organism-level biomedical system research since they can facilitate deep optical imaging by reducing light scattering in tissue and then enable three-dimensional signal visualization and quantification of tissues. While the previously reported optical clearing methods have addressed some of six key issues (i.e. transparency, efficiency, reproducibility, preservation of emission from fluorescence proteins, preservation of membrane integrity, and the ease of operation), none has yet addressed all of them. Here, we present a new, convenient, inexpensive and reproducible approach to optical clearing, termed UbasM, providing unprecedented performance in terms of clearing rate, the ease of operation and satisfactory fluorescence protein/membrane integrity preservation while achieving sufficient transparency to permit 3D volumetric imaging.

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Citation Download Citation

Lingling Chen, Guiye Li, Yingchao Li, Lina Liu, Ang Liu, and Shuangchen Ruan "UbasM: a simple, rapid, efficient balanced optical clearing method for brain imaging", Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104810W (12 February 2018); https://doi.org/10.1117/12.2283223

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