Novel fiber optic-based needle redox imager for cancer diagnosis

Udayakumar Kanniyappan; He N. Xu; Qinggong Tang; Brandon Gaitan; Yi Liu; Lin Z. Li; Yu Chen

doi:10.1117/12.2293133

26 February 2018 Novel fiber optic-based needle redox imager for cancer diagnosis

Udayakumar Kanniyappan, He N. Xu, Qinggong Tang, Brandon Gaitan, Yi Liu, Lin Z. Li, Yu Chen

Proceedings Volume 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis; 104890J (2018) https://doi.org/10.1117/12.2293133
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Despite various technological advancements in cancer diagnosis, the mortality rates were not decreased significantly. We aim to develop a novel optical imaging tool to assist cancer diagnosis effectively. Fluorescence spectroscopy/imaging is a fast, rapid, and minimally invasive technique which has been successfully applied to diagnosing cancerous cells/tissues. Recently, the ratiometric imaging of intrinsic fluorescence of reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), as pioneered by Britton Chance and the co-workers in 1950-70’s, has gained much attention to quantify the physiological parameters of living cells/tissues. The redox ratio, i.e., FAD/(FAD+NADH) or FAD/NADH, has been shown to be sensitive to various metabolic changes in in vivo and in vitro cells/tissues. Optical redox imaging has also been investigated for providing potential imaging biomarkers for cancer transformation, aggressiveness, and treatment response. Towards this goal, we have designed and developed a novel fiberoptic-based needle redox imager (NRI) that can fit into an 11G clinical coaxial biopsy needle for real time imaging during clinical cancer surgery. In the present study, the device is calibrated with tissue mimicking phantoms of FAD and NADH along with various technical parameters such as sensitivity, dynamic range, linearity, and spatial resolution of the system. We also conducted preliminary imaging of tissues ex vivo for validation. We plan to test the NRI on clinical breast cancer patients. Once validated this device may provide an effective tool for clinical cancer diagnosis.

Conference Presentation

Citation Download Citation

Udayakumar Kanniyappan, He N. Xu, Qinggong Tang, Brandon Gaitan, Yi Liu, Lin Z. Li, and Yu Chen "Novel fiber optic-based needle redox imager for cancer diagnosis", Proc. SPIE 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis, 104890J (26 February 2018); https://doi.org/10.1117/12.2293133

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available