Intraoperative margin assessment with photoacoustic molecular imaging (Conference Presentation)

Katheryne E. Wilson; Juergen K. Willmann

doi:10.1117/12.2290746

15 March 2018 Intraoperative margin assessment with photoacoustic molecular imaging (Conference Presentation)

Katheryne E. Wilson, Juergen K. Willmann

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104942E (2018) https://doi.org/10.1117/12.2290746
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Intraoperative tumor margin assessment during breast-conserving surgical resection is critically needed as positive margins are found in up to 34% of patients. Spectroscopic photoacoustic (sPA) molecular imaging combined with translatable antibody (Ab)-indocyanine green (ICG) contrast agent targeted to B7-H3, a molecular marker differentially expressed in breast cancer, may aid in margin assessment in murine breast cancers. ICG was conjugated to anti-B7-H3 antibodies through standard NHS chemistry. FVB/N Tg(MMTV/PyMT634Mul mice, a transgenic mouse model for breast cancer development, 5 weeks of age with ductal carcinoma in situ (DCIS) or small invasive carcinomas were given 33 μg of B7-H3-ICG 3-5 days before surgical resection. During excision of sequential sections of the lower mammary glands, fluorescence, multi-wavelength (680-900 nm, 10 nm increments) sPA, and B-mode ultrasound imaging were performed. Section specific molecular B7-H3 signal was compared to fluorescence imaging and histological (H&E) analysis. B-mode US and sPA imaging signal were able to relay accurate anatomical and molecular tissue information. sPA molecular imaging was able to detect B7-H3-ICG uptake in DCIS and early invasive carcinomas less than 1 mm in diameter. Furthermore, histological analysis of the excised tissues was able to show strong correlation between sPA imaging signal and disease state and location. While fluorescence imaging was able to detect ICG signal, there was insufficient resolution and specificity to identify and aid in resection of small cancerous foci. Adding molecular sPA imaging to help guide intraoperative margin assessment may increase the occurrence of negative margins which will decrease local disease recurrence.

Conference Presentation

Citation Download Citation

Katheryne E. Wilson and Juergen K. Willmann "Intraoperative margin assessment with photoacoustic molecular imaging (Conference Presentation)", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942E (15 March 2018); https://doi.org/10.1117/12.2290746

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KEYWORDS

Breast cancer

Molecular imaging

Signal detection

Luminescence

Photoacoustic spectroscopy

Molecular spectroscopy

Tissues

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