Fluorescence lifetime imaging of microviscosity changes during ER autophagy in live cells

Ying He; Soham Samanta; Wanjun Gong; Wufan Liu; Wenhui Pan; Zhigang Yang; Junle Qu II

doi:10.1117/12.2293440

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19 February 2018 Fluorescence lifetime imaging of microviscosity changes during ER autophagy in live cells

Ying He, Soham Samanta, Wanjun Gong, Wufan Liu, Wenhui Pan, Zhigang Yang, Junle Qu II

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Proceedings Volume 10495, Biophotonics and Immune Responses XIII; 104950R (2018) https://doi.org/10.1117/12.2293440
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Unfolded or misfolded protein accumulation inside Endoplasmic Reticulum (ER) will cause ER stress and subsequently will activate cellular autophagy to release ER stress, which would ultimately result in microviscosity changes. However, even though, it is highly significant to gain a quantitative assessment of microviscosity changes during ER autophagy to study ER stress and autophagy behaviors related diseases, it has rarely been reported yet. In this work, we have reported a BODIPY based fluorescent molecular rotor that can covalently bind with vicinal dithiols containing nascent proteins in ER and hence can result in ER stress through the inhibition of the folding of nascent proteins. The change in local viscosity, caused by the release of the stress in cells through autophagy, was quantified by the probe using fluorescence lifetime imaging. This work basically demonstrates the possibility of introducing synthetic chemical probe as a promising tool to diagnose ER-viscosity-related diseases.

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Ying He, Soham Samanta, Wanjun Gong, Wufan Liu, Wenhui Pan, Zhigang Yang, and Junle Qu II "Fluorescence lifetime imaging of microviscosity changes during ER autophagy in live cells", Proc. SPIE 10495, Biophotonics and Immune Responses XIII, 104950R (19 February 2018); https://doi.org/10.1117/12.2293440

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