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20 February 2018Indices of polarimetric purity for biological tissues inspection
Albert Van Eeckhout,1 Angel Lizana,1 Enric Garcia-Caurelhttps://orcid.org/0000-0001-8202-7810,2 José J. Gil,3 Adrià Sansa,1 Carla Rodríguez,1 Irene Estévez,1 Emilio González,4,5 Juan C. Escalera,1 Ignacio Moreno,6 Juan Campos1
1Univ. Autònoma de Barcelona (Spain) 2LPICM, CNRS, École Polytechnique, Univ. Paris-Saclay (France) 3Univ. de Zaragoza (Spain) 4Univ. Autónoma de Madrid (Spain) 5Hospital Univ. de Canarias (Spain) 6Univ. Miguel Hernández de Elche (Spain)
We highlight the interest of using the Indices of Polarimetric Purity (IPPs) for the biological tissue inspection. These are three polarimetric metrics focused on the study of the depolarizing behaviour of the sample. The IPPs have been recently proposed in the literature and provide different and synthetized information than the commonly used depolarizing indices, as depolarization index (PΔ) or depolarization power (Δ). Compared with the standard polarimetric images of biological samples, IPPs enhance the contrast between different tissues of the sample and show differences between similar tissues which are not observed using the other standard techniques. Moreover, they present further physical information related to the depolarization mechanisms inherent to different tissues. In addition, the algorithm does not require advanced calculations (as in the case of polar decompositions), being the indices of polarimetric purity fast and easy to implement. We also propose a pseudo-coloured image method which encodes the sample information as a function of the different indices weights. These images allow us to customize the visualization of samples and to highlight certain of their constitutive structures. The interest and potential of the IPP approach are experimentally illustrated throughout the manuscript by comparing polarimetric images of different ex-vivo samples obtained with standard polarimetric methods with those obtained from the IPPs analysis. Enhanced contrast and retrieval of new information are experimentally obtained from the different IPP based images.
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Albert Van Eeckhout, Angel Lizana, Enric Garcia-Caurel, José J. Gil, Adrià Sansa, Carla Rodríguez, Irene Estévez, Emilio González, Juan C. Escalera, Ignacio Moreno, Juan Campos, "Indices of polarimetric purity for biological tissues inspection ," Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104971V (20 February 2018); https://doi.org/10.1117/12.2290283