Spatially confined photoinactivation of bacteria: towards novel tools for detailed mechanistic studies

Hanna Thomsen; Jeemol James; Anne Farewell; Marica B. Ericson

doi:10.1117/12.2290718

23 February 2018 Spatially confined photoinactivation of bacteria: towards novel tools for detailed mechanistic studies

Hanna Thomsen, Jeemol James, Anne Farewell, Marica B. Ericson

Proceedings Volume 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII; 1049825 (2018) https://doi.org/10.1117/12.2290718
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Antimicrobial resistance is a serious global threat fueling an accelerated field of research aimed at developing novel antimicrobial therapies. A particular challenge is the treatment of microbial biofilms formed upon bacterial growth and often associated with chronic infections. Biofilms comprise bacteria that have adhered to a surface and formed 3D microcolonies, and demonstrate significantly increased antimicrobial resistance compared to the planktonic counterpart. A challenge in developing novel strategies for fighting these chronic infections is a lack of mechanistic understanding of what primarily contributes to enhanced drug resistance. Tools for noninvasive study of live biofilms are necessary to begin to understand these mechanisms on both a single cell and 3D level.

Herein, a method by which multiphoton microscopy is implemented to study a biofilm model of Staphylococcus epidermidis to noninvasively visualize and measure penetration of compounds in 3D biofilm structure and two photon excitation was exploited for spatially confined photoinactivation and microscopy optimized for evaluation of microbiological viability at a microscopic level. Future studies are aimed at future development of the proposed techniques for detailed studies of, e.g., quorum sensing and mechanisms contributing to antimicrobial resistance.

Citation Download Citation

Hanna Thomsen, Jeemol James, Anne Farewell, and Marica B. Ericson "Spatially confined photoinactivation of bacteria: towards novel tools for detailed mechanistic studies", Proc. SPIE 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII, 1049825 (23 February 2018); https://doi.org/10.1117/12.2290718

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Bacteria

Resistance

Two photon excitation microscopy

Luminescence

Visualization

3D image processing

3D modeling

Show All Keywords

Keywords/Phrases

Search In:

Publication Years