Single image structured illumination (SISIM) for in-vivo imaging

Zachary R. Hoffman; Kose Kivanc; Charles A. DiMarzio

doi:10.1117/12.2285699

23 February 2018 Single image structured illumination (SISIM) for in-vivo imaging

Zachary R. Hoffman, Kose Kivanc, Charles A. DiMarzio

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 1049918 (2018) https://doi.org/10.1117/12.2285699
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Recently, it has been demonstrated that sectioning within a turbid media using structured illumination microscopy can be greatly enhanced through the use of a Hilbert transform algorithm. This technique, known as single image structured illumination microscopy (SISIM), provides high quality sectioning even within a strongly refracting/scattering medium, such as tissue. It is found that motion artifacts comprise a large portion of the error in in-vivo imaging. Thus, we expand on the SISIM technique by implementing an image registration algorithm which greatly mitigates motion induced artifacts. Multiple images and movies are developed to demonstrate SISIMs ability to provide real-time, in-vivo imaging of human tissue.

Citation Download Citation

Zachary R. Hoffman, Kose Kivanc, and Charles A. DiMarzio "Single image structured illumination (SISIM) for in-vivo imaging", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049918 (23 February 2018); https://doi.org/10.1117/12.2285699

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